GNA14 stimulation of KLF7 promotes malignant growth of endometrial cancer through upregulation of HAS2

Abstract Background Endometrial cancer (UCEC) is one of the most common gynecological malignancies. We previously found that overexpression G protein ? subunit 14 (GNA14) promoted UCEC growth. Krüppel-like factor 7 (KLF7) acts as an oncogene in various types, whereas connection between GNA14 and KLF7 unclear. herein explored involvement GNA14/KLF7 development. Methods Clinical relevance GNA14, HAS2 was analyzed from TCGA by immunohistochemical staining. Knockdown indicated genes were conducted transfecting cells with siRNAs lentivirus, respectively. mRNA expression detected qRT-PCR Western blot. CCK8, colony formation, cell cycle, apoptosis, transwell wound healing performed to check biology function vitro. Tumor growth nude mice vivo function. RNA sequencing used determine dys-regulated genes. Results demonstrated stimulated cells. There a positive correlation normal tissues. In vitro, proliferation, cycle progression, migration Apoptosis inhibited KLF7. Xenografted tumorigenesis suppressed knockdown. Furthermore, results showed regulated large amount genes, among which hyaluronan synthase 2 (HAS2) downregulated knockdown Based on database immunoblotting assays, positively Lastly, reversed oncogenic role migration, xenografted tumor Conclusion Taken together, we reveal GNA14/KLF7/HAS2 signaling cascade exerts promoting during